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  4. Method to extract and use time-resolved ion clouds from a spectrometric data set
 
patent

Method to extract and use time-resolved ion clouds from a spectrometric data set

Koolen, Cedric David  
•
Koolen, Sven R.J
2025

A method is proposed for extracting single-particle and/or single-cell events from a spectrometric data set comprising intensity values. A respective intensity value is proportional to the number of ions collected by a detector in a given time interval. The method comprises: grouping (22) the intensity values into sets of groups of consecutive intensity values, a respective set being <b>characterised by</b> a distinct group size of consecutive intensity values; determining (23) a threshold value separating a background from candidate event intensity values; calculating (24) a dissolved intensity as a mean of the intensity values forming a background signal; carrying out (25) a first candidate event identification by obtaining candidate peak groups based on a feature characterising a respective group; carrying (26) out a second candidate event identification by obtaining ion cloud groups based on the threshold value; carrying out (27) a true event identification by comparing the first and second candidate event identifications to obtain one or more ion cloud group events for a respective group size; and summing (28) the intensity values of a respective ion cloud group event and subtracting the dissolved intensity as scaled from the summed intensity values of the respective ion cloud group event to obtain a total intensity value per particle or cell.

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Type
patent
EPO Family ID

89224768

Additional titles

Procédé d'extraction et d'utilisation de nuages d'ions à résolution temporelle à partir d'un ensemble de données spectrométriques

Author(s)
Koolen, Cedric David  

EPFL

Koolen, Sven R.J
Issuers

École Polytechnique Fédérale de Lausanne

Note

Alternative title(s) : (de) Verfahren zur extraktion und verwendung zeitaufgelöster ionenwolken aus einem spektrometrischen datensatz (fr) Procédé d'extraction et d'utilisation de nuages d'ions à résolution temporelle à partir d'un ensemble de données spectrométriques

TTO classification

6.2251.1

EPFL units
AVP-R-TTO  
LMER  
IdentifierCountry codeKind codeDate issued

WO2025133766

WO

A1

2025-06-26

EP4576162

EP

A1

2025-06-25

Available on Infoscience
October 8, 2025
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/254808
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