Quantitative imaging of species-specific lipid transport in mammalian cells - endocytosis inhibitors
To test the predominant role of the non-vesicular route for retrograde transport in U-2 OS cells, we inhibited vesicular trafficking using Brefeldin A, which blocks COPI coat formation at Golgi membranes and Wortmannin, which broadly affects endosome formation and trafficking and quantified transport of SM(Y), PE(18:1/Y), PC(Y:16:0), PC(Y/20:4) using the lipid imaging pipeline.
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Iglesias‐Artola, J. M., Böhlig, K., Schuhmann, K., Cook, K. C., Lennartz, H. M., Schuhmacher, M. M., Barahtjan, P., López, C. J., Šachl, R., Garikapati, V., Pombo‐García, K., Lohmann, A., Riegerová, P., Hof, M., Drobot, B., Shevchenko, A., Honigmann, A., & Nadler, A. (2025). Quantitative imaging of species-specific lipid transport in mammalian cells - endocytosis inhibitors [Dataset]. In BioStudies Database. EMBL-EBI. https://doi.org/10.6019/s-biad1695
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Max Planck Institute of Molecular Cell Biology and Genetics
Max Planck Institute of Molecular Cell Biology and Genetics
Max Planck Institute of Molecular Cell Biology and Genetics
Max Planck Institute of Molecular Cell Biology and Genetics
Max Planck Institute of Molecular Cell Biology and Genetics
EPFL
Max Planck Institute of Molecular Cell Biology and Genetics
Czech Academy of Sciences, J. Heyrovský Institute of Physical Chemistry
Max Planck Institute of Molecular Cell Biology and Genetics
2025-03
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