Candida albicans Adhesion Measured by Optical Nanomotion Detection
Cellular adhesion plays an important role in numerous fundamental physiological and pathological processes. Its measurement is relatively complex, requires sophisticated equipment, and, in most cases, cannot be carried out without breaking the links between the studied cell and its target. In this contribution, we propose a novel, nanomotion-based, technique that overcomes these drawbacks. The applied force is generated by the studied cell itself (nanomotion), whereas cellular movements are detected by traditional optical microscopy and dedicated software. The measurement is non-destructive, single-cell sensitive, and permits following the evolution of the adhesion as a function of time. We applied the technique on different strains of the fungal pathogen Candida albicans on a fibronectin-coated surface. We demonstrated that this novel approach can significantly simplify, accelerate, and make more affordable living cells-substrate adhesion measurements.
WOS:001109410100001
2023-11-01
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Funder | Grant Number |
Belgian Federal Science Policy Office (Belspo) | |
European Space Agency | |
Research Foundation-Flanders (FWO) | AUGE/13/19 |
FWO-SNSF | 310030L_197946 |
Swiss National Science Foundation (SNSF) | CRSII5_173863 |
Agence Nationale de Recherche | ANR-10-LABX-62-IBEID |