The localization of electromagnetic interference (EMI) sources is of high importance in electromagnetic compatibility applications. Recently, a novel localization technique based on the time-reversal cavity (TRC) concept was proposed using only one sensor, and its application to localize EMI sources was validated numerically. In this paper, we present a validation of the proposed time-reversal process in which the forward step of the time-reversal process is performed experimentally and the backward step is carried out via numerical simulations, a realistic scenario which is applicable to practical source localization problems. To the best of the authors’ knowledge, this is the first implementation of a three-dimensional electromagnetic time-reversal process in which the forward signal is provided experimentally while the backward propagation step is carried out numerically. The considered experimental setup is formed by a partially open cavity and two monopole antennas to emulate the EMI source and the sensor (receiving antenna), respectively. Assuming that the location of the source is the feed point of the monopole antenna, the resulting three-dimensional location error in the experimental validation was only 1.49 cm, which is about one-third the length of the monopole antenna, corresponding to about λmin/2 (diffraction limit).