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research article
Chemogenetic Control of Nanobodies
February 17, 2020
Engineered nanobody allows reversible control of activity in cells through the binding of small molecules.
We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows interrogation of their roles in basic biological processes, an approach that should be applicable to numerous previously described GFP fusions. We also outline how the binding affinities of other nanobodies can be controlled by small molecules.
Type
research article
Web of Science ID
WOS:000514052200001
Authors
•
Tarnawski, Miroslaw
•
Mueller, Thorsten G.
•
Otsuka, Shotaro
•
Hiblot, Julien
•
Koch, Birgit
•
Kueblbeck, Moritz
•
Kraeusslich, Hans-Georg
•
Ellenberg, Jan
•
Publication date
2020-02-17
Publisher
Published in
Volume
17
Start page
279
End page
282
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
March 5, 2020
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