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book part or chapter
Merging of image data in electron crystallography
Electron crystallography of membrane proteins uses cryo-transmission electron microscopy to record images and diffraction patterns of frozen-hydrated 2D crystals. Each two-dimensional (2D) crystal is only imaged once, at one specific tilt angle, and the recorded images can be automatically processed with the 2dx/MRC software package. Processed image data from non-tilted and tilted 2D crystals then need to be merged into a 3D reconstruction of the membrane protein structure. We here describe the process of the 3D merging, using the 2dx software system.
Type
book part or chapter
Authors
Arheit, Marcel
•
Castano-Diez, Daniel
•
Thierry, Raphael
•
Abeyrathne, Priyanka
•
Gipson, Bryant R
•
Publication date
2012-10-07
Publisher
Published in
Electron Crystallography of Soluble and Membrane Proteins
Start page
195
End page
209
Volume
955
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
February 13, 2020
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