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research article

Digital confocal microscopy through a multimode fiber

Loterie, Damien
•
Farahi, Salma  
•
Papadopoulos, Ioannis  
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2015
Optics Express

Acquiring high-contrast optical images deep inside biological tissues is still a challenging problem. Confocal microscopy is an important tool for biomedical imaging since it improves image quality by rejecting background signals. However, it suffers from low sensitivity in deep tissues due to light scattering. Recently, multimode fibers have provided a new paradigm for minimally invasive endoscopic imaging by controlling light propagation through them. Here we introduce a combined imaging technique where confocal images are acquired through a multimode fiber. We achieve this by digitally engineering the excitation wavefront and then applying a virtual digital pinhole on the collected signal. In this way, we are able to acquire images through the fiber with significantly increased contrast. With a fiber of numerical aperture 0.22, we achieve a lateral resolution of 1.5 mu m, and an axial resolution of 12.7 mu m. The point-scanning rate is currently limited by our spatial light modulator (20Hz). (C)2015 Optical Society of America

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Type
research article
DOI
10.1364/Oe.23.023845
Web of Science ID

WOS:000362419900077

Author(s)
Loterie, Damien
Farahi, Salma  
Papadopoulos, Ioannis  
Goy, Alexandre  
Psaltis, Demetri  
Moser, Christophe  
Date Issued

2015

Publisher

Optical Soc Amer

Published in
Optics Express
Volume

23

Issue

18

Start page

23845

End page

23858

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LAPD  
LO  
Available on Infoscience
December 2, 2015
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/121258
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