Repository logo

Infoscience

  • English
  • French
Log In
Logo EPFL, École polytechnique fédérale de Lausanne

Infoscience

  • English
  • French
Log In
  1. Home
  2. Academic and Research Output
  3. Journal articles
  4. Adaptive optics enables multimode 3D super-resolution microscopy via remote focusing
 
research article

Adaptive optics enables multimode 3D super-resolution microscopy via remote focusing

Navikas, Vytautas  
•
Descloux, Adrien Charles  
•
Grussmayer, Kristin Stefanie  
Show more
June 10, 2021
Nanophotonics

A variety of modern super-resolution micro-scopy methods provide researchers with previouslyinconceivable biological sample imaging opportunities ata molecular resolution. All of these techniques excel atimaging samples that are close to the coverslip, howeverimaging at large depths remains a challenge due to aber-rations caused by the sample, diminishing the resolution ofthe microscope. Originating in astro-imaging, the adaptiveoptics (AO) approach for wavefront shaping using adeformable mirror is gaining momentum in modern mi-croscopy as a convenient approach for wavefront control.AO has the ability not only to correct aberrations but alsoenables engineering of the PSF shape, allowing localiza-tion of the emitter axial position over several microns. Inthis study, we demonstrate remote focusing as another AO benefit for super-resolution microscopy. We show theability to record volumetric data (45×45×10 μm), whilekeeping the sample axially stabilized using a standard widefield setup with an adaptive optics addon. We processed the data with single-molecule localization routines and/or computed spatiotemporal correlations, demonstrating subdiffraction resolution.

  • Files
  • Details
  • Metrics
Type
research article
DOI
10.1515/nanoph-2021-0108
Author(s)
Navikas, Vytautas  
Descloux, Adrien Charles  
Grussmayer, Kristin Stefanie  
Marion, Sanjin  
Radenovic, Aleksandra  
Date Issued

2021-06-10

Published in
Nanophotonics
Volume

10

Issue

9

Start page

2451

End page

2458

Note

This is an Open Access article under the terms of the Creative Commons Attribution License

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LBEN  
Available on Infoscience
June 15, 2021
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/178877
Logo EPFL, École polytechnique fédérale de Lausanne
  • Contact
  • infoscience@epfl.ch

  • Follow us on Facebook
  • Follow us on Instagram
  • Follow us on LinkedIn
  • Follow us on X
  • Follow us on Youtube
AccessibilityLegal noticePrivacy policyCookie settingsEnd User AgreementGet helpFeedback

Infoscience is a service managed and provided by the Library and IT Services of EPFL. © EPFL, tous droits réservés