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  4. Intracellular Recording of Cardiomyocyte Action Potentials with Nanopatterned Volcano-Shaped Microelectrode Arrays
 
research article

Intracellular Recording of Cardiomyocyte Action Potentials with Nanopatterned Volcano-Shaped Microelectrode Arrays

Desbiolles, B. X. E.  
•
de Coulon, E.
•
Bertsch, A.  
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August 19, 2019
Nano Letters

Micronanotechnology-based multielectrode ar- rays have led to remarkable progress in the field of transmembrane voltage recording of excitable cells. However, providing long-term optoporation- or electroporation-free intracellular access remains a considerable challenge. In this study, a novel type of nanopatterned volcano-shaped micro- electrode (nanovolcano) is described that spontaneously fuses with the cell membrane and permits stable intracellular access. The complex nanostructure was manufactured following a simple and scalable fabrication process based on ion beam etching redeposition. The resulting ring-shaped structure provided passive intracellular access to neonatal rat cardiomyocytes. Intracellular action potentials were successfully recorded in vitro from different devices, and continuous recording for more than 1 h was achieved. By reporting transmembrane action potentials at potentially high spatial resolution without the need to apply physical triggers, the nanovolcanoes show distinct advantages over multielectrode arrays for the assessment of electrophysiological characteristics of cardiomyocyte networks at the transmembrane voltage level over time.

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Type
research article
DOI
10.1021/acs.nanolett.9b02209
Author(s)
Desbiolles, B. X. E.  
de Coulon, E.
Bertsch, A.  
Rohr, S.
Renaud, P.  
Date Issued

2019-08-19

Publisher

American Chemical Society (ACS)

Published in
Nano Letters
Volume

19

Issue

9

Start page

6173

End page

6181

Subjects

Intracellular electrophysiology

•

transmembrane access

•

microelectrode arrays

•

continuous recording

•

cell−electrode interface

•

nanoscale patterning

•

neonatal rat cardiomyocytes

•

nanovolcano

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LMIS4  
FunderGrant Number

FNS

200021-175943

FNS

310030- 169234

Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/161142
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