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research article

High-fidelity optical diffraction tomography of multiple scattering samples

Lim, Joowon  
•
Ayoub, Ahmed B.
•
Antoine, Elizabeth E.
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September 11, 2019
Light: Science & Applications

We propose an iterative reconstruction scheme for optical diffraction tomography that exploits the split-step nonparaxial (SSNP) method as the forward model in a learning tomography scheme. Compared with the beam propagation method (BPM) previously used in learning tomography (LT-BPM), the improved accuracy of SSNP maximizes the information retrieved from measurements, relying less on prior assumptions about the sample. A rigorous evaluation of learning tomography based on SSNP (LT-SSNP) using both synthetic and experimental measurements confirms its superior performance compared with that of the LT-BPM. Benefiting from the accuracy of SSNP, LT-SSNP can clearly resolve structures that are highly distorted in the LT-BPM. A serious limitation for quantifying the reconstruction accuracy for biological samples is that the ground truth is unknown. To overcome this limitation, we describe a novel method that allows us to compare the performances of different reconstruction schemes by using the discrete dipole approximation to generate synthetic measurements. Finally, we explore the capacity of learning approaches to enable data compression by reducing the number of scanning angles, which is of particular interest in minimizing the measurement time.

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Type
research article
DOI
10.1038/s41377-019-0195-1
Web of Science ID

WOS:000485866900001

Author(s)
Lim, Joowon  
Ayoub, Ahmed B.
Antoine, Elizabeth E.
Psaltis, Demetri  
Date Issued

2019-09-11

Publisher

Springer

Published in
Light: Science & Applications
Volume

8

Start page

82

Subjects

Optics

•

finite-difference method

•

image-reconstruction

•

blood-cells

•

principles

•

intensity

Note

This article is licensed under a Creative Commons Attribution 4.0 International License

Editorial or Peer reviewed

REVIEWED

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Available on Infoscience
October 1, 2019
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/161701
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