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  4. Background Rejection in Two-Photon Fluorescence Image Scanning Microscopy
 
research article

Background Rejection in Two-Photon Fluorescence Image Scanning Microscopy

Sheppard, Colin J. R.
•
Castello, Marco
•
Tortarolo, Giorgio  
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May 22, 2023
Photonics

We discuss the properties of signal strength and integrated intensity in two-photon excitation confocal microscopy and image scanning microscopy. The resolution, optical sectioning and background rejection are all improved over nonconfocal two-photon microscopy. Replacing the pinhole of confocal two-photon microscopy with a detector array increases the peak intensity of the point spread function. The outer pixels of a detector array give signals from defocused regions, and thus the processing of these, such as through subtraction, can further improve optical sectioning and background rejection.

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