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research article
Background Rejection in Two-Photon Fluorescence Image Scanning Microscopy
May 22, 2023
We discuss the properties of signal strength and integrated intensity in two-photon excitation confocal microscopy and image scanning microscopy. The resolution, optical sectioning and background rejection are all improved over nonconfocal two-photon microscopy. Replacing the pinhole of confocal two-photon microscopy with a detector array increases the peak intensity of the point spread function. The outer pixels of a detector array give signals from defocused regions, and thus the processing of these, such as through subtraction, can further improve optical sectioning and background rejection.
Type
research article
Web of Science ID
WOS:001011310700001
Authors
Sheppard, Colin J. R.
•
Castello, Marco
•
•
Zunino, Alessandro
•
Slenders, Eli
•
Bianchini, Paolo
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Vicidomini, Giuseppe
•
Diaspro, Alberto
Publication date
2023-05-22
Publisher
Published in
Volume
10
Issue
5
Start page
601
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
July 3, 2023
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