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  4. Cell shape changes indicate a role for extrinsic tensile forces in Drosophila germ-band extension
 
research article

Cell shape changes indicate a role for extrinsic tensile forces in Drosophila germ-band extension

Butler, Lucy C.
•
Blanchard, Guy B.
•
Kabla, Alexandre J.
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2009
Nature Cell Biology

Drosophila germ-band extension (GBE) is an example of the convergence and extension movements that elongate and narrow embryonic tissues. To understand the collective cell behaviours underlying tissue morphogenesis, we have continuously quantified cell intercalation and cell shape change during GBE. We show that the fast, early phase of GBE depends on cell shape change in addition to cell intercalation. In antero-posterior patterning mutants such as those for the gap gene Kruppel, defective polarized cell intercalation is compensated for by an increase in antero-posterior cell elongation, such that the initial rate of extension remains the same. Spatio-temporal patterns of cell behaviours indicate that an antero-posterior tensile force deforms the germ band, causing the cells to change shape passively. The rate of anteroposterior cell elongation is reduced in twist mutant embryos, which lack mesoderm. We propose that cell shape change contributing to germ-band extension is a passive response to mechanical forces caused by the invaginating mesoderm.

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Type
research article
DOI
10.1038/ncb1894
Web of Science ID

WOS:000267603100015

Author(s)
Butler, Lucy C.
Blanchard, Guy B.
Kabla, Alexandre J.
Lawrence, Nicola J.
Welchman, David P.
Mahadevan, L.
Adams, Richard J.
Sanson, Benedicte
Date Issued

2009

Published in
Nature Cell Biology
Volume

11

Start page

859

End page

U181

Subjects

Epithelial Morphogenesis

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Gastrulation

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Intercalation

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Rearrangement

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Invagination

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Melanogaster

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Elongation

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Divisions

•

Notochord

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Polarity

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
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Available on Infoscience
November 30, 2010
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/60084
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