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research article

Microfluidic-assisted bioprinting of tissues and organoids at high cell concentrations

Serex, Ludovic  
•
Sharma, Kunal  
•
Rizov, Victor
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April 1, 2021
Biofabrication

Despite its simplicity, which makes it the most commonly used bioprinting method today, extrusion-based bioprinting suffers from its inability to reproduce the complex tissue architecture found in organs. Generally, this printing method allows for the dispensing of solutions of a predefined cell concentration through a rudimentary needle. Moreover, to avoid cell lysis in the dispensing needle, which is detrimental to the viability of the printed tissue, as well as cell loss in dead volumes of tubing, thereby increasing the cost of printing tissue, a common strategy has been to print with cell concentrations much lower in comparison to the concentrations found in living tissues. As a result, cell-to-cell distance is increased in the dispensed samples impairing communication through cytokines. Here, we present a microfluidic-based print head capable of modulating the printed cell concentration in real-time. This device allows bioprinting at high cell concentrations by concentrating and dispensing fibroblasts at concentrations up to 10 million cells center dot mL(-1). We also demonstrate that this device can be used to print bladder organoids. As the cell seeding concentration is of major importance for organogenesis in 3D culture, organoid printing allows the user to standardize the process of organoid formation and achieve more reliable and reproducible results.

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Type
research article
DOI
10.1088/1758-5090/abca80
Web of Science ID

WOS:000628213600001

Author(s)
Serex, Ludovic  
Sharma, Kunal  
Rizov, Victor
Bertsch, Arnaud  
McKinney, John D.  
Renaud, Philippe  
Date Issued

2021-04-01

Publisher

IOP PUBLISHING LTD

Published in
Biofabrication
Volume

13

Issue

2

Article Number

025006

Subjects

Engineering, Biomedical

•

Materials Science, Biomaterials

•

Engineering

•

Materials Science

•

microfluidics

•

bioprinting

•

organoid

•

bladder

•

concentrator

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

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LSBI  
LMIS4  
Available on Infoscience
April 10, 2021
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/177096
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