Within the scope of a broader research project about visual and thermal interaction effects on human comfort and perception, the aim of this paper is to study the variation of thermal, visual and overall comfort votes of people exposed to blue and orange glazing. The study, conducted in a controlled test room and involving a total of 75 participants, is repeated at three temperature levels to investigate whether variations in comfort votes are affected by the thermal environment. Results show that participants changed their comfort votes for both thermal and overall comfort, beside the expected visual comfort, due to changes in glazing colour. Larger variations in thermal and overall comfort votes are observed in the close-to-comfortable temperature range (22 °C). Temperature-related effects can be seen for visual and thermal comfort evaluations. Overall comfort shows a positive correlation with both visual and thermal comfort.