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  4. Effects of methotrexate on transfected DNA stability in mammalian cells
 
research article

Effects of methotrexate on transfected DNA stability in mammalian cells

Pallavicini, M. G.
•
DeTeresa, P. S.
•
Rosette, C.
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1990
Molecular and cellular biology

The chromosomal locations, amounts, and level of expression of transfected, amplified c-myc and dihydrofolate reductase sequences were measured in cells cultured in the presence and absence of methotrexate. These studies show that the location and amount of transfected sequences, as well as the level of expression, were more variable when the cells were cultured in methotrexate.

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Type
research article
DOI
10.1128/MCB.10.1.401
PubMed ID

2403643

Author(s)
Pallavicini, M. G.
DeTeresa, P. S.
Rosette, C.
Gray, J. W.
Wurm, F. M.  
Date Issued

1990

Published in
Molecular and cellular biology
Volume

10

Issue

1

Start page

401

End page

4

Subjects

Animals

•

DNA Probes

•

DNA

•

Recombinant/genetics

•

Gene Amplification/*drug effects

•

Gene Expression Regulation/drug effects

•

Methotrexate/*pharmacology

•

Mice

•

Nucleic Acid Hybridization

•

Proto-Oncogene Proteins/genetics

•

Proto-Oncogene Proteins c-myc

•

Proto-Oncogenes

•

Tetrahydrofolate Dehydrogenase/genetics

•

Transfection

Note

Biomedical Sciences Division, Lawrence Livermore National Laboratory, Livermore, California 94550.

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LBTC  
Available on Infoscience
June 5, 2007
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/7621
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