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research article

Visible spectrum extended-focus optical coherence microscopy for label-free sub-cellular tomography

Marchand, Paul James  
•
Bouwens, Arno  
•
Szlag, Daniel
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2017
Biomedical Optics Express

We present a novel extended-focus optical coherence microscope (OCM) attaining 0.7 μm axial and 0.4 μm lateral resolution maintained over a depth of 40 μm, while preserving the advantages of Fourier domain OCM. Our system uses an ultra-broad spectrum from a supercontinuum laser source. As the spectrum spans from near-infrared to visible wavelengths (240 nm in bandwidth), we call the system visOCM. The combination of such a broad spectrum with a high-NA objective creates an almost isotropic 3D submicron resolution. We analyze the imaging performance of visOCM on microbead samples and demonstrate its image quality on cell cultures and ex-vivo brain tissue of both healthy and alzheimeric mice. In addition to neuronal cell bodies, fibers and plaques, visOCM imaging of brain tissue reveals fine vascular structures and sub-cellular features through its high spatial resolution. Sub-cellular structures were also observed in live cells and were further revealed through a protocol traditionally used for OCT angiography.

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Type
research article
DOI
10.1364/BOE.8.003343
Web of Science ID

WOS:000404738300014

Author(s)
Marchand, Paul James  
Bouwens, Arno  
Szlag, Daniel
Nguyen, David  
Descloux, Adrien  
Sison, Miguel  
Coquoz, Severine
Extermann, Jérôme
Lasser, Theo  
Date Issued

2017

Published in
Biomedical Optics Express
Volume

8

Issue

7

Start page

3343

End page

3359

Subjects

Optical Coherence Tomography

•

Optical Coherence Microscopy

•

High resolution imaging

•

Three-dimensional imaging

•

Cellular imaging

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

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Available on Infoscience
August 2, 2017
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/139512
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