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  4. A bimetallic nanocoral Au decorated with Pt nanoflowers (bio)sensor for H<sub>2</sub>O<sub>2</sub> detection at low potential
 
research article

A bimetallic nanocoral Au decorated with Pt nanoflowers (bio)sensor for H2O2 detection at low potential

Sanzò, Gabriella
•
Taurino, Irene  
•
Puppo, Francesca  
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2017
Methods

In this work, we have developed for the first time a method to make novel gold and platinum hybrid bimetallic nanostructures differing in shape and size. Au-Pt nanostructures were prepared by electrodeposition in two simple steps. The first step consists of the electrodeposition of nanocoral Au onto a gold substrate using hydrogen as a dynamic template in an ammonium chloride solution. After that, the Pt nanostructures were deposited onto the nanocoral Au organized in pores. Using Pt (II) and Pt (IV), we realized nanocoral Au decorated with Pt nanospheres and nanocoral Au decorated with Pt nanoflowers, respectively. The bimetallic nanostructures showed better capability to electrochemically oxidize hydrogen peroxide compared with nanocoral Au. Moreover, Au-Pt nanostructures were able to lower the potential of detection and a higher performance was obtained at a low applied potential. Then, glucose oxidase was immobilized onto the bimetallic Au-Pt nanostructure using cross-linking with glutaraldehyde. The biosensor was characterized by chronoamperometry at +0.15 V vs. Ag pseudo-reference electrode (PRE) and showed good analytical performances with a linear range from 0.01 to 2.00 mM and a sensitivity of 33.66 µA/mM cm2. The good value of Kmapp (2.28 mM) demonstrates that the hybrid nanostructure is a favorable environment for the enzyme. Moreover, the low working potential can minimize the interference from ascorbic acid and uric acid as well as reducing power consumption to effect sensing. The simple procedure to realize this nanostructure and to immobilize enzymes, as well as the analytical performances of the resulting devices, encourage the use of this technology for the development of biosensors for clinical analysis.

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Type
research article
DOI
10.1016/j.ymeth.2017.06.005
Web of Science ID

WOS:000413613500011

Author(s)
Sanzò, Gabriella
Taurino, Irene  
Puppo, Francesca  
Antiochia, Riccarda
Gorton, Lo
Favero, Gabriele
Mazzei, Franco
Carrara, Sandro  
De Micheli, Giovanni  
Date Issued

2017

Publisher

Academic Press Inc Elsevier Science

Published in
Methods
Volume

129

Issue

October

Start page

89

End page

95

Subjects

Au nanocoral

•

Pt nanoflowers

•

Pt nanospheres

•

Bimetallic hybrid nanostructure

•

Glucose oxidase

•

Hydrogen peroxide

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LSI1  
Available on Infoscience
October 17, 2017
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/141475
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