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research article

Comparison between SOFI and STORM

Geissbühler, Stefan  
•
Dellagiacoma, Claudio  
•
Lasser, Theo  
2011
Biomedical Optics Express

A straightforward method to achieve super-resolution consists of taking an image sequence of stochastically blinking emitters using a standard wide-field fluorescence microscope. Densely packed single molecules can be distinguished sequentially in time using high-precision localization algorithms (e.g., PALM and STORM) or by analyzing the statistics of the temporal fluctuations (SOFI). In a face-to-face comparison of the two post-processing algorithms, we show that localization-based super-resolution can deliver higher resolution enhancements but imposes significant constraints on the blinking behavior of the probes, which limits its applicability for live-cell imaging. SOFI, on the other hand, works more consistently over different photo-switching kinetics and also delivers information about the specific blinking statistics. Its suitability for low SNR acquisition reveals SOFI's potential as a high-speed super-resolution imaging technique.

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Type
research article
DOI
10.1364/BOE.2.000408
Web of Science ID

WOS:000299875200001

Author(s)
Geissbühler, Stefan  
Dellagiacoma, Claudio  
Lasser, Theo  
Date Issued

2011

Published in
Biomedical Optics Express
Volume

2

Issue

3

Start page

408

End page

420

Subjects

Superresolution

•

Fluorescence microscopy

Note

This paper was published in Biomedical Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://www.opticsinfobase.org/boe/abstract.cfm?uri=boe-2-3-408. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.

Editorial or Peer reviewed

REVIEWED

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Available on Infoscience
February 2, 2011
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/63856
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