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review article

Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology

Laohakunakorn, Nadanai  
•
Grasemann, Laura  
•
Lavickova, Barbora  
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March 24, 2020
Frontiers In Bioengineering And Biotechnology

Cell-free systems offer a promising approach to engineer biology since their open nature allows for well-controlled and characterized reaction conditions. In this review, we discuss the history and recent developments in engineering recombinant and crude extract systems, as well as breakthroughs in enabling technologies, that have facilitated increased throughput, compartmentalization, and spatial control of cell-free protein synthesis reactions. Combined with a deeper understanding of the cell-free systems themselves, these advances improve our ability to address a range of scientific questions. By mastering control of the cell-free platform, we will be in a position to construct increasingly complex biomolecular systems, and approach natural biological complexity in a bottom-up manner.

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Type
review article
DOI
10.3389/fbioe.2020.00213
Web of Science ID

WOS:000531529800001

Author(s)
Laohakunakorn, Nadanai  
Grasemann, Laura  
Lavickova, Barbora  
Michielin, Gregoire  
Shahein, Amir  
Swank, Zoe  
Maerkl, Sebastian J.  
Date Issued

2020-03-24

Published in
Frontiers In Bioengineering And Biotechnology
Volume

8

Start page

213

Subjects

Biotechnology & Applied Microbiology

•

Multidisciplinary Sciences

•

Science & Technology - Other Topics

•

cell-free synthetic biology

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cell-free protein synthesis

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in vitro reconstitution

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microfluidics

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compartmentalization

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artificial cell

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in vitro replication

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free protein-synthesis

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directed peptide-synthesis

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transcription-translation system

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amino acid incorporation

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simian virus-40 dna

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in-vitro synthesis

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escherichia-coli

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gene-expression

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chemical communication

•

functional proteins

Note

This article is licensed under a Creative Commons Attribution 4.0 International License

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LBNC  
Available on Infoscience
May 22, 2020
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/168870
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