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research article

Wide-field time-gated SPAD imager for phasor-based FLIM applications

Ulku, Arin  
•
Ardelean, Andrei  
•
Antolovic, Michel  
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February 5, 2020
Methods and Applications in Fluorescence

We describe the performance of a new wide area time-gated single-photon avalanche diode (SPAD) array for phasor-FLIM, exploring the effect of gate length, gate number and signal intensity on the measured lifetime accuracy and precision. We conclude that the detector functions essentially as an ideal shot noise limited sensor and is capable of video rate FLIM measurement. The phasor approach used in this work appears ideally suited to handle the large amount of data generated by this type of very large sensor (512 × 512 pixels), even in the case of small number of gates and limited photon budget.

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Type
research article
DOI
10.1088/2050-6120/ab6ed7
Author(s)
Ulku, Arin  
Ardelean, Andrei  
Antolovic, Michel  
Weiss, Shimon
Charbon, Edoardo  
Bruschini, Claudio  
Michalet, Xavier
Date Issued

2020-02-05

Publisher

IOP

Published in
Methods and Applications in Fluorescence
Volume

8

Issue

2

Article Number

024002

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

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March 14, 2020
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/167286
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