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research article

Cell viability assessment by flow cytometry using yeast as cell model

Mernier, Guillaume  
•
Piacentini, Niccolò
•
Tornay, Raphaël
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2011
Sensors and Actuators B: Chemical

This paper reports the new combination of cell sorting and counting capabilities on a single device. Most state-of-the-art devices combining these technologies use optical techniques requiring complicated experimental setups and labeled samples. The use of a label-free, electrical device significantly decreases the system complexity and makes it more appropriate for use in point-of-care diagnostics. Living and dead yeast cells are separated by dielectrophoretic forces and counted using coulter counters. The combination of these two methods allows the determination of the percentage of living and dead cells for viability studies of cell samples. The device could further be used for sorting and counting of blood cells in applications such as diagnosis of insufficient cell concentrations, identification of cell deficiencies or bacterial contamination. The use of dielectrophoresis (DEP) as sorting principle allows to separate cells based on their dielectric properties in the place of size-based separation, enabling sorting of large panels of cells and separation of infected and non-infected cells of the same type.

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Type
research article
DOI
10.1016/j.snb.2009.11.066
Web of Science ID

WOS:000291195200014

Author(s)
Mernier, Guillaume  
Piacentini, Niccolò
Tornay, Raphaël
Buffi, Nina  
Renaud, Philippe  
Date Issued

2011

Publisher

Elsevier

Published in
Sensors and Actuators B: Chemical
Volume

154

Issue

2

Start page

160

End page

163

Subjects

Cell sorting

•

Cell counting

•

Dielectrophoresis

•

Impedance measurements

•

Label-free

•

Viability study

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LMIS4  
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/67460
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