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  4. Epithelial-Mesenchymal Transition-Derived Cells Exhibit Multi-Lineage Differentiation Potential Similar to Mesenchymal Stem Cells
 
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research article

Epithelial-Mesenchymal Transition-Derived Cells Exhibit Multi-Lineage Differentiation Potential Similar to Mesenchymal Stem Cells

Battula, Venkata Lokesh
•
Evans, Kurt William
•
Hollier, Brett George
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2010
STEM CELLS

The epithelial-to-mesenchymal transition (EMT) is an embryonic process that becomes latent in most normal adult tissues. Recently, we have shown that induction of EMT endows breast epithelial cells with stem cell traits. In this report, we have further characterized the EMT-derived cells and shown that these cells are similar to mesenchymal stem cells (MSCs) with the capacity to differentiate into multiple tissue lineages. For this purpose, we induced EMT by ectopic expression of Twist, Snail or TGF- in immortalized human mammary epithelial cells (HMECs). We found that the EMT-derived cells and MSCs share many properties including the antigenic profile typical of MSCs, i.e. CD44+, CD24- and CD45-. Conversely, MSCs express EMT-associated genes, such as Twist, Snail and FOXC2. Interestingly, CD140b (PDGFR-), a marker for naive MSCs, is exclusively expressed in EMT-derived cells and not in their epithelial counterparts. Moreover, functional analyses revealed that EMT-derived cells but not the control cells can differentiate into Alizarin Red S-positive mature osteoblasts, Oil Red O-positive adipocytes and Alcian Blue-positive chondrocytes similar to MSCs. We also observed that EMT-derived cells but not the control cells invade and migrate towards MDA-MB-231 breast cancer cells similar to MSCs. In vivo wound homing assays in nude mice revealed that the EMT-derived cells home to wound sites similar to MSCs. In conclusion, we have demonstrated that the EMT-derived cells are similar to MSCs in gene expression, multi-lineage differentiation, and ability to migrate towards tumor cells and wound sites.

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Type
research article
DOI
10.1002/stem.467
Web of Science ID

WOS:000281566200013

Author(s)
Battula, Venkata Lokesh
•
Evans, Kurt William
•
Hollier, Brett George
•
Shi, Yuexi
•
Marini, Frank C.
•
Ayyanan, Ayyakkannu
•
Wang, Rui-Yu
•
Brisken, Cathrin  
•
Guerra, Rudy
•
Andreeff, Michael
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Date Issued

2010

Published in
STEM CELLS
Start page

1435

End page

1445

Subjects

Epithelial-mesenchymal transition

•

Twist

•

Snail

•

MSC

•

mesenchymal stem cells

•

CD140b

•

PDGFR-beta

Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
UPBRI  
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/51527
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