We propose and experimentally demonstrate the possibility to use a pump signal based on bipolar pulse sequences using single-sideband suppressed-carrier (SSB-SC) modulation in Brillouin optical time-domain analysis (BOTDA) sensors. The SSB-SC modulated pump makes a sequential use of the Brillouin gain and loss spectra, increasing the intensity contrast of the measurements. The method is demonstrated using bipolar Golay codes along a 50 km sensing fiber and 2 m spatial resolution. Results indicate that the use of bipolar sequences provides a higher SNR enhancement and stronger robustness to pump depletion in comparison to BOTDA systems employing conventional unipolar sequences.