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research article

A biphasic growth model for cell pole elongation in mycobacteria

Hannebelle, Mélanie T. M.  
•
Ven, Joëlle X. Y.  
•
Toniolo, Chiara  
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January 23, 2020
Nature Communications

Mycobacteria grow by inserting new cell wall material in discrete zones at the cell poles. This pattern implies that polar growth zones must be assembled de novo at each division, but the mechanisms that control the initiation of new pole growth are unknown. Here, we combine time-lapse optical and atomic force microscopy to measure single-cell pole growth in mycobacteria with nanometer-scale precision. We show that single-cell growth is biphasic due to a lag phase of variable duration before the new pole transitions from slow to fast growth. This transition and cell division are independent events. The difference between the lag and interdivision times determines the degree of single-cell growth asymmetry, which is high in fast-growing species and low in slow-growing species. We propose a biphasic growth model that is distinct from previous unipolar and bipolar models and resembles “new end take off” (NETO) dynamics of polar growth in fission yeast.

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Type
research article
DOI
10.1038/s41467-019-14088-z
Author(s)
Hannebelle, Mélanie T. M.  
Ven, Joëlle X. Y.  
Toniolo, Chiara  
Eskandarian, Haig A.  
Vuaridel-Thurre, Gaëlle  
McKinney, John D.  
Fantner, Georg E.  
Date Issued

2020-01-23

Publisher

Nature Research

Published in
Nature Communications
Volume

11

Start page

452

Note

This is an Open Access article under the terms of the Creative Commons Attribution License

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

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UPKIN  
LBNI  
Available on Infoscience
February 10, 2020
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/165229
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