Lactate is an important metabolite in human body and, among possible medical applications, it can be used to monitor physical activity. Actually, its concentration represents a clear indication whether optimal training intensity is kept or if muscles are under anaerobic conditions. Routine procedures to measure lactate concentration during physical activity are represented by invasive measurements, which require blood sampling from the patient or the athlete. So, a great advantage would be derived by the possibility to monitor this analyte using non-invasive techniques. Considering the possibility to measure lactate in human sweat during sport activities, this paper presents the characterization of saline aqueous solutions containing sodium lactate by means of Electrochemical Impedance Spectroscopy (EIS). Measurements were performed using a two-electrode electrochemical cell and acquired spectra were analyzed also by means of equivalent electrical circuit (EEC) modeling. Results show an effect due to lactate concentration on solution impedance in the high-frequency region of spectrum, where a change in solution resistance is measured. At the same time, no changes in the measured capacitance were found. Future work will study the electrochemical behavior of lactate solutions also at higher frequencies to further investigate the possible use of EIS for lactate levels monitoring during sport activities.