A dielectrophoretic detection device comprising a chip, with a flow channel having at least one inlet and one outlet, and at least a detection area configured to detect analytes trapped on functionalised beads flowing within said flow channel, a first and a second electrode assembly shaped as rows of parallel pillars extending over said height of the flow channel, configured to generate under an appropriate electric tension therebetween an electric field so as to form an electrical barrier, preventing said beads to cross said barrier and drawing said beads to said detection area by dielectrophoretic forces, where they are clustered and concentrated.The device may be provided with multiple rows of parallel pillars of electrode assemblies extending over the height of the flow channel, forming multiple concentration lines. The flow channel may be further provided with further rows of parallel pillars of electrode assemblies crossing said flow channel in transverse direction, forming further incubation lines.