Protein synthesis- and fucosylation-dependent mechanisms in corticosterone facilitation of long-term memory in the chick
Long-term memory formation for a passive avoidance task in day-old chicks, which requires a late phase of glycoprotein fucosylation (5-8 hr posttraining), is dependent on a corticosterone action in the brain. In addition, corticosterone enhances late-phase fucosylation. In this study, the authors explored (a) to what extent the memory-facilitating action of corticosterone is dependent on protein fucosylation and (b) whether a protein synthesis mechanism might be involved in the steroid effects on memory and late-phase fucosylation. A combination of psychopharmacological and biochemical experiments, including the fucosylation inhibitor 2-deoxygalactose (2-DG), the protein synthesis inhibitor anisomycin (ANI), and radiolabeled fucose, indicated that the late phase of glycoprotein synthesis involved in the memory-facilitating effect of corticosterone occurs on newly synthesized proteins.
Keywords: Animals ; Anisomycin/pharmacology ; Avoidance Learning/drug effects/ physiology ; Brain/drug effects/ physiology ; Brain Mapping ; Chickens/ physiology ; Corticosterone/ physiology ; Fucose/ metabolism/pharmacology ; Injections ; Membrane Glycoproteins ; Mental Recall/drug effects/physiology ; Myelin-Associated Glycoprotein/ biosynthesis ; Nerve Tissue Proteins/ biosynthesis ; Protein Processing ; Post-Translational/drug effects/physiology ; Protein Synthesis Inhibitors/pharmacology ; Retention (Psychology)/drug effects/ physiology ; Transcription ; Genetic/drug effects/physiology
Author address: Behavior Research Group, Open University, Milton Keynes, United Kingdom. firstname.lastname@example.org
Record created on 2007-01-18, modified on 2016-08-08