Production of lactate using crabtree negative organisms expressing recombinant lactate dehydrogenase genes

Crabtree neg. organisms such as Kluyveromyces, Pichia, Hansenula and Candida, are used to make selected org. products such as lactic acid. The organisms are cultured in a first culture medium that includes glucose, under conditions that promote cellular respiration. The organisms are then cultured under a second set of conditions that promote prodn. of the selected org. product. The organisms preferably contain an exogenous lactate dehydrogenase gene. Thus, gene PDC1 encoding pyruvate decarboxylase in Kluyveromyces marxianus was disrupted by site-directed mutagenesis. The LDH gene from Kluyveromyces thermotolerans under control of the Saccharomyces cerevisiae PDC1 promoter was then introduced to this Kluyveromyces marxianus strain by electroporation. The resulting strain was able to produce lactic acid using aerobic conditions for cell growth and anaerobic conditions for lactate prodn. [on SciFinder (R)]

    Keywords: crabtree neg yeast lactic acid fermn recombinant


    Can 138:3760

    16-5 FIELD Section Title:Fermentation and Bioindustrial Chemistry


    Field url:



    Application: US





    19990521 FIELD Citations:Baniel; US 4275234 A 1981|de Louvencourt; US 4806472 A 1989|Hollenberg; US 4859596 A 1989|van Den Berg; US 4943529 A 1990|Baniel; US 5510526 A 1996|Sarhaddar; US 5641406 A 1997|Doquin; US 5712152 A 1998|Wood; US 5783423 A 1998|Fowlkes; US 5789184 A 1998|Ligon; US 5817502 A 1998|Eyal; US 5831122 A 1998|Zhang; US 5843760 A 1998|Badziong; US 5866371 A 1999|Hallborn; US 5866382 A 1999|Fowlkes; US 5876951 A 1999|Cho; US 5882884 A 1999|Anon; EP 0370160 A2 1990|Anon; WO 9300440 1993|Anon; WO 9400554 1994|Anon; WO 9401569 1994|Anon; WO 9716528 1997|Anon; WO 9826079 1998|Anon; WO 9854337 1998|Anon; WO 9914335 1999|Anon; WO 0014258 2000|Porro; Applied and Environmental Microbiology 1999, 65, 4211|Franzblau; Mycopathologia 1983, 82, 185|Passoth; Applied Biochemistry and Biotechnology 1996, 57/58, 201|Porro, D; Biotechnol Prog 1995, 11, 294|Stewart, J; Biotechnology and Genetic Engineering Reviews 1997, 14, 67|Urk, H; Applied and Environmental Microbiology 1990, 56, 281|Anon; TheYeasts, A Taxonomic Study, Fourth Edition 1998, 240|Anon; Yeast Physiology and Biotechnology, Chapter 5, 203|Adachi, E; Journal of Fermentation and Bioengineering 1998, 86, 284|Becker, D; Methods in Enzymology 1991, 194, 182|Bianchi, M; Molecular Microbiology 1996, 19, 27|Billard, P; Gene 1995, 162, 93|Brambilla, L; Advances in Bioprocess Engineering 1994, 417|Cho, J; Applied And Environmental Microbiology 1998, 64, 1350|Danners, H; Applied Biochemistry and Biotechnology 1998, 70-72, 887|Dequin, S; Bio/Technology 1994, 12, 173|Destruelle, M; Yeast 1999, 15, 361|Durrens, P; Current Genetics 1990, 18, 7|Flikweert, M; Yeast 1996, 12, 247|Flikweert, M; Applied And Environmental Microbiology 1997, 3399|Garmyn, D; Applied And Environmental Microb I ology 1995, 61, 266|Gonzalez, F; Yeast 1997, 13, 1399|Gunge, N; Journal of Bacteriology 1981, 145, 382|Gunge, N; European Journal of Epidemiology 1988, 4, 409|Hohmann, S; Mol Gen Genet 1993, 241, 657|Hohmann, S; Journal of Bacteriology 1991, 173, 7963|Hsieh, H; Appl Microbiol Biotechnol 1998, 49, 147|Hsieh, H; Appl Microbiol Biotechnol 1998, 49, 411|Ito, H; Journal of Bacteriology 1983, 153, 163|Kiers, J; Yeast 1998, 14, 459|Lighthelm, M; Appl Microbiol Biotechnol 1988, 29, 67|Morsomme, P; The EMBO Journal 1996, 15, 5513|Seeboth, P; Journal of Bacteriology 1990, 172, 678|Shi, N; Appl Microbiol Biotechnol 1998, 50, 339|Siso, M; FEBS Letters 1996, 387, 7|Subden, R; Canadian Journal of Microbiology 1982, 28, 883|Ullrich, J; Methods in Enzymology Volume XVIII Vitamins and Coenzymes Part A 1970, 109|van Hoek, P; Applied And Environmental Microbiology 1998, 64, 2133|Warnecke, D; The Journal of Biological Chemistry 1999, 274, 13048|Wesolowski-Louvel, M; Nonconventional Yeasts in Biotechnology, A Handbook, Chapter 5 1996, 139|Witte, V; J Basic Microbiol 1989, 29, 707|Zeeman, A; Microbiology 1998, 144, 3437


    Record created on 2007-01-11, modified on 2016-08-08


  • There is no available fulltext. Please contact the lab or the authors.

Related material