Inverse metabolic engineering: a strategy for directed genetic engineering of useful phenotypes
A review. The classical method of metabolic engineering, identifying a rate-detg. step in a pathway and alleviating the bottleneck by enzyme overexpression, has motivated much research but has enjoyed only limited practical success. Intervention of other limiting steps, of counter-balancing regulation, and of unknown coupled pathways often confounds this direct approach. Here the concept of inverse metabolic engineering is codified and its application is illustrated with several examples. Inverse metabolic engineering means the elucidation of a metabolic engineering strategy by: first, identifying, constructing, or calcg. a desired phenotype; second, detg. the genetic or the particular environmental factors conferring that phenotype; and third, endowing that phenotype on another strain or organism by directed genetic or environmental manipulation. This paradigm has been successfully applied in several contexts, including elimination of growth factor requirements in mammalian cell culture and increasing the energetic efficiency of microaerobic bacterial respiration. [on SciFinder (R)]
vh-2002-1.pdf
restricted
343.33 KB
Adobe PDF
a93bb465e0c1758392cfb77fdfa65aa8
vh-2002-4.pdf
restricted
454.94 KB
Adobe PDF
23ab02b06db626105f863b617d53c188