Ultrasensitive reporter protein detection in genetically engineered bacteria

We demonstrate the use of laser-induced. fluorescence confocal spectroscopy to measure analyte-stimulated enhanced green fluorescent protein (egfp) synthesis by genetically modified Escherichia coli bioreporter cells. Induction is measured in cell lysates and, since the spectroscopic focal volume is approximately the size of one bioreporter cell, also in individual live bacteria. This is, to our knowledge, the first ever proof-of-concept work utilizing instrumentation with single-molecule detection capability to monitor bioreporter response. Although we use arsenic inducible bioreporters here, The method is extensible to gfp/egfp bioreporters that are responsive to other substances.


Published in:
Analytical Chemistry, 77, 9, 2683-2689
Year:
2005
ISSN:
0003-2700
Keywords:
Laboratories:




 Record created 2006-10-03, last modified 2018-07-07


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