Ultrasensitive reporter protein detection in genetically engineered bacteria

• Published in: Analytical Chemistry (ISSN: 0003-2700), vol. 77, num. 9, p. 2683-2689
• Publication date: 2005

We demonstrate the use of laser-induced. fluorescence confocal spectroscopy to measure analyte-stimulated enhanced green fluorescent protein (egfp) synthesis by genetically modified Escherichia coli bioreporter cells. Induction is measured in cell lysates and, since the spectroscopic focal volume is approximately the size of one bioreporter cell, also in individual live bacteria. This is, to our knowledge, the first ever proof-of-concept work utilizing instrumentation with single-molecule detection capability to monitor bioreporter response. Although we use arsenic inducible bioreporters here, The method is extensible to gfp/egfp bioreporters that are responsive to other substances.

Keywords: GREEN FLUORESCENT PROTEIN ; CELL SENSING SYSTEMS ; WHOLE-CELL ; SINGLE ; MOLECULES ; ESCHERICHIA-COLI ; BIOSENSORS ; ARSENITE ; SENSOR ; SPECTROSCOPY ; TOLUENE

Reference

• LOB-ARTICLE-2005-015
• doi:10.1021/ac048127k
• View record in Web of Science

Record created on 2006-10-03, modified on 2017-05-10