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research article

Ultrasensitive reporter protein detection in genetically engineered bacteria

Wells, M.
•
Gösch, M.
•
Rigler, R.  
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2005
Analytical Chemistry

We demonstrate the use of laser-induced. fluorescence confocal spectroscopy to measure analyte-stimulated enhanced green fluorescent protein (egfp) synthesis by genetically modified Escherichia coli bioreporter cells. Induction is measured in cell lysates and, since the spectroscopic focal volume is approximately the size of one bioreporter cell, also in individual live bacteria. This is, to our knowledge, the first ever proof-of-concept work utilizing instrumentation with single-molecule detection capability to monitor bioreporter response. Although we use arsenic inducible bioreporters here, The method is extensible to gfp/egfp bioreporters that are responsive to other substances.

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Type
research article
DOI
10.1021/ac048127k
Web of Science ID

WOS:000228877500013

Author(s)
Wells, M.
Gösch, M.
Rigler, R.  
Harms, H.  
Lasser, T.  
van der Meer, J. R.
Date Issued

2005

Published in
Analytical Chemistry
Volume

77

Issue

9

Start page

2683

End page

2689

Subjects

GREEN FLUORESCENT PROTEIN

•

CELL SENSING SYSTEMS

•

WHOLE-CELL

•

SINGLE

•

MOLECULES

•

ESCHERICHIA-COLI

•

BIOSENSORS

•

ARSENITE

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SENSOR

•

SPECTROSCOPY

•

TOLUENE

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
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Available on Infoscience
October 3, 2006
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/234940
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