Infoscience

Journal article

Uv-ozone ashing of cells and tissues for spatially resolved trace element analysis

UV/ozone ashing of thin tissue sections and cell cultures is a simple technique to enhance relative elemental concentrations, while maintaining their spatial location at the sub-micron level. This approach may enhance the capability of spatially resolved analysis techniques to detect the distribution of trace elements in biological matrices. We present results from light microscopy and x-ray spectromicroscopy studies of tissues and cells demonstrating that the micro-structure is very well conserved. We show the signal enhancement resulting from the removal of carbon, which allows otherwise undetectable gadolinium to be mapped in cancer tissue for a novel neutron capture therapy.

    Keywords: ashing ; microchemical analysis ; trace element detection ; spectromicroscopy ; SPECTROMICROSCOPY ; MEPHISTO ; CULTURES ; SPECTROMICROSCOPY

    Note:

    Ecole Polytech Fed Lausanne, PH Ecublens, Inst Phys Appl, CH-1015 Lausanne, Switzerland. Univ Wisconsin, Ctr Synchrotron Radiat, Stoughton, WI 53589 USA. CNR, Ist Neurobiol, I-00137 Rome, Italy. Paul Scherrer Inst, CH-5232 Villigen, Switzerland. CNR, Ist Struttura Mat, Rome, Italy. Univ Wisconsin, Dept Phys, Madison, WI 53706 USA. Gilbert, B, Ecole Polytech Fed Lausanne, PH Ecublens, Inst Phys Appl, CH-1015 Lausanne, Switzerland.

    ISI Document Delivery No.: 360JG

    Reference

    Record created on 2006-10-03, modified on 2016-08-08

Fulltext

  • There is no available fulltext. Please contact the lab or the authors.

Related material