Photoemission analysis of chemical differences between the membrane and cytoplasm of neuronal cells
We demonstrate the possibility of using x-ray photoemission spectroscopy (XPS) to distinguish between the chemical properties of the neuron membrane and of the cytoplasm in brain cells. This was possible by analysing cells de-capped by using a newly developed technique. The XPS spectra clearly show chemical differences between the cell cytoplasm and the membrane, for example between nitrogen in -NH2 and -NH3, which are characteristic of the proteins and membrane phospholipids, respectively. A preliminary (and not conclusive) interpretation of all the observed spectroscopic features is given.
Cnr,ist struttura mat,i-00044 frascati,italy. cnr,ist biol cellulare,i-00137 rome,italy. cnr,ist neurobiol,i-00137 rome,italy. Lorusso, GF, ECOLE POLYTECH FED LAUSANNE,INST PHYS APPL,CH-1015 LAUSANNE,SWITZERLAND.
ISI Document Delivery No.: XH160
Record created on 2006-10-03, modified on 2016-08-08