Imaging cells and tissues with refractive index radiology
Can individual cells, including live cells, be imaged using hard x rays? Common wisdom until now required sophisticated staining techniques for this task. We show instead that individual cells and cell details can be detected in culture solution and tissues with no staining and no other contrast-enhancing preparation. The sample examined can be much thicker than for many other microscopy techniques without sacrificing the capability to resolve cells. The key factor in our approach is the use of a coherent synchrotron source and of contrast mechanisms based on the refractive index. The first successful tests were conducted on a variety of cell systems including skin and internal leaf cells, mouse neurons, rabbit fibroblast cells, and human tumor cells.
WOS:000225426700053
2004
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Pohang Univ Sci & Technol, Dept Mat Sci & Engn, Pohang, South Korea. Acad Sinica, Inst Phys, Taipei, Taiwan. Pandis Biomed Res Assoc, Chupei, Taiwan. Mackay Mem Hosp, Mackay Med Nursing & Management Coll, Dept Med Res, Taipei, Taiwan. Mackay Mem Hosp, Mackay Med Nursing & Management Coll, Dept Internal Med, Taipei, Taiwan. Taipei Med Univ, Taipei, Taiwan. Natl Univ Singapore, Dept Mat Sci, Singapore 0511, Singapore. Natl Univ Singapore, Singapore MIT Alliance, Singapore 0511, Singapore. Natl Univ Singapore, Dept Pharm, Singapore 0511, Singapore. Natl Univ Singapore, Singapore Synchrotron Light Source, Singapore 0511, Singapore. Ecole Polytech Fed Lausanne, Fac Sci Base, CH-1015 Lausanne, Switzerland. Je, JH, Pohang Univ Sci & Technol, Dept Mat Sci & Engn, Pohang, South Korea. jhje@postech.ac.kr
ISI Document Delivery No.: 875NH
REVIEWED
EPFL