Toward a molecular genetic analysis of spermatogenesis in Drosophila melanogaster: characterization of male-sterile mutants generated by single P element mutagenesis
We describe 83 recessive autosomal male-sterile mutations, generated by single P element mutagenesis in Drosophila melanogaster. Each mutation has been localized to a lettered subdivision of the polytene map. Reversion analyses, as well as complementation tests using available chromosomal deficiencies, indicate that the insertions are responsible for the mutant phenotypes. These mutations represent 63 complementation groups, 58 of which are required for spermatogenesis. Phenotypes of the spermatogenesis mutants were analyzed by light microscopy. Mutations in 12 loci affect germline proliferation, spermatocyte growth, or meiosis. Mutations in 46 other loci disrupt differentiation and maturation of spermatids into motile sperm. This collection of male-sterile mutants provides the basis for a molecular genetic analysis of spermatogenesis.
- URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8244010
Keywords: Animals ; Cell Differentiation ; Chromosome Mapping ; Crosses ; Genetic ; Drosophila melanogaster/genetics/*physiology ; Female ; Fertility ; Genes ; Recessive ; Genetic Complementation Test ; Genetic Markers ; In Situ Hybridization ; Infertility ; Male/genetics ; Male ; Meiosis/genetics ; *Mutagenesis ; Insertional ; Phenotype ; Research Support ; Non-U.S. Gov't ; Research Support ; U.S. Gov't ; P.H.S. ; Salivary Glands/cytology ; Spermatogenesis/*genetics ; Spermatozoa/*cytology
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235-9038.
Record created on 2006-08-24, modified on 2016-08-08