Biophysical approaches to G protein-coupled receptors: Structure, function and dynamics

• Published in: Journal of Computer-Aided Molecular Design, vol. 13, num. 3, p. 209-219
• Publication date: 1999

G protein-coupled receptors (GPCR) represent a large family of drug targets for which there is no high-resolution structural information. In order to understand the mechanisms of ligand recognition and receptor activation, there is a strong need for novel biophysical methods. In this perspective we provide an overview of recent experimental approaches used to explore the molecular architecture and dynamics of GPCR and their interactions with ligands and G proteins using biophysical, non-crystallographic, methods.

Keywords: Electron paramagnetic resonance (EPR) ; Fluorescence resonance energy transfer (FRET) ; Ligand-receptor interactions ; Neurokinin receptors ; Nonsense suppression mutagenesis ; Rhodopsin ; drug ; guanine nucleotide binding protein ; drug receptor binding

Reference

• EPFL-ARTICLE-88458
• doi:10.1023/A:1008052002695

Record created on 2006-08-14, modified on 2017-05-12