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  4. Structure and partial amino acid sequence of calf thymus DNA topoisomerase II: Comparison with other type II enzymes
 
research article

Structure and partial amino acid sequence of calf thymus DNA topoisomerase II: Comparison with other type II enzymes

Austin, C. A.
•
Barot, H. A.
•
Margerrison, E. E. C.
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1990
Biochemical and Biophysical Research Communications

The partial amino acid sequence of p140 calf thymus DNA topoisomerase II was determined by analysis of cyanogen bromide peptides. Five peptides were aligned and shared extensive homology with sequences derived from cDNA clones for the human topoisomerase II isoenzyme forms. Less homology was seen with the Drosophila, yeast and bacterial type II enzymes. Calf and human enzymes shared epitopes allowing isolation of a cDNA clone to human topoisomerase II isoenzyme alpha. Our results indicate that calf thymus p140 topoisomerase II is an active N-terminal proteolytic fragment of the native p180 enzyme and demonstrate that mammalian type II enzymes exhibit close sequence similarity.

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Type
research article
DOI
10.1016/0006-291X(90)92156-T
Author(s)
Austin, C. A.
Barot, H. A.
Margerrison, E. E. C.
Turcatti, G.  
Wingfield, P.
Hayes, M. V.
Fisher, L. M.
Date Issued

1990

Published in
Biochemical and Biophysical Research Communications
Volume

170

Issue

2

Start page

763

End page

768

Subjects

dna topoisomerase (atp hydrolysing)

•

DNA Topoisomerases

•

Type II

Editorial or Peer reviewed

REVIEWED

Written at

OTHER

EPFL units
PTCB  
Available on Infoscience
August 14, 2006
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/232854
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