Formation of halogenated medium chain hydrocarbons by a lipoxygenase/hydroperoxide halolyase-mediated transformation in planktonic microalgae

A rapid transformation of eicosapentaenoic acid is initiated upon disintegration of cells of the marine diatom Stephanopyxis turris. In contrast to any other fatty acid cleaving activity known, this diatom releases a blend of chlorinated C8-hydrocarbons in addition to an unsaturated 12-oxo-acid. Using labeled precursors, trapping experiments and selective inhibition of enzyme activities we were able to show that a lipoxygenase activates the C20 fatty acid to a hydroperoxide, which is subsequently cleaved by a new hydroperoxide halolyase to the C8- and C12-products. This adds a fundamentally new pathway to the limited set of halogenating enzymatic activities known from nature.

Published in:
Journal of the American Chemical Society, 128, 22, 7114-7115
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 Record created 2006-07-05, last modified 2020-07-30

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