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  4. Enzymic reaction in a three-phase liquid-liquid-solid catalyst system. Application to the optical resolution of phenylalanine
 
research article

Enzymic reaction in a three-phase liquid-liquid-solid catalyst system. Application to the optical resolution of phenylalanine

Crelier, Simon
•
Renken, Albert  
•
Flaschel, Erwin
1991
Swiss Chem

The development of a phasic system for the enzymic resoln. of racemic phenylalanine (I) up to benchtop pilot plant scale is described. The principle is based upon the hydrolysis of racemic Pr ester of I with immobilized a-chymotrypsin (II) in which only the L-isomer reacts to give enantiomerically pure L-I. By employing Me2CHCH2COMe and H2O as solvents, several problems inherent to the monophasic system (such as substrate autohydrolysis, dimerization, or pptn. at high pH or severe pH decreases at the activity optimum of the enzyme (5.5-6.5) due to low buffer capacity) are overcome, since the immiscible org. phase partially exts. the neutral form of the ester, thus shifting the buffer capacity to optimal reaction values. 100% Conversions are thereby obtained without the necessity of external pH control. Various data are presented for monophasic, biphasic, and triphasic (ie. with immobilized II) systems, including conversions, substrate partitions between the 2 solvents, and reaction kinetics. Although batch expts. with the 3-phase system were successful in a continuously stirred tank reactor, in continuous operation in a plug flow reactor conversion did not exceed 80%, probably a result of mass transfer limitation between both liq. phases. [on SciFinder (R)]

  • Details
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Type
research article
Author(s)
Crelier, Simon
Renken, Albert  
Flaschel, Erwin
Date Issued

1991

Published in
Swiss Chem
Volume

13

Issue

1

Start page

7

End page

10, 12

Subjects

Kinetics (of chymotrypsin and chymotrypsin immobilized deriv.); Resolution (of phenylalanine

•

by chymotrypsin hydrolysis of Pr esters in triphasic system)

•

phenylalanine resoln immobilized chymotrypsin triphasic system

Note

CAN 115:6960

16-5

Fermentation and Bioindustrial Chemistry

Inst. Genie Chim.,Ec. Polytech. Fed. Lausanne,Lausanne,Switz.

Journal

written in English.

54966-38-0 Role: PRP (Properties) (partition of, between isobutylmethyl ketone and water); 63-91-2 (Phenylalanine) Role: RCT (Reactant), RACT (Reactant or reagent) (resoln. of, by chymotrypsin hydrolysis of Pr esters in biphasic system); 9004-07-3 (Chymotrypsin) Role: BIOL (Biological study) (a, immobilized, phenylalanine resoln. by hydrolysis of Pr esters with, in triphasic system)

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LGRC  
Available on Infoscience
April 18, 2006
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/229374
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