Enzymic ester hydrolysis in liquid two-phase systems for the preparation of L-phenylalanine
L-Phenylalanine was produced from phenylalanine Pr esters by chymotrypsin in 2-phase systems (org. phase using iso-Bu Me ketone and aq. phase). Control of pH during the enzymic reaction was crit. The reaction was carried out in a batch fermentor or a fixed-bed reactor. It was also possible to use chymotrypsin immobilized in 3-aminopropyltriethoxysilane and(or) glutaraldehyde-activated silica gel. [on SciFinder (R)]
1988
542
Enzyme Eng. 9
219
23
CAN 111:55734
16-2
Fermentation and Bioindustrial Chemistry
Inst. Chem. Eng.,Swiss Fed. Inst. Technol.,Lausanne,Switz.
Journal
written in English.
121661-48-1 Role: RCT (Reactant), RACT (Reactant or reagent) (hydrolysis of, by chymotrypsin in liq. 2-phase system); 9004-07-3 (Chymotrypsin) Role: RCT (Reactant), RACT (Reactant or reagent) (phenylalanine ester hydrolysis by, in liq. 2-phase system); 63-91-2P (L-Phenylalanine) Role: PREP (Preparation) (prepn. of, by enzymic ester hydrolysis in liq. 2-phase system)
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