A photometric test for the cyclization activity of cyclodextrin-glycosyltransferases

A quick and reliable test was developed to measure the cyclization activity of cyclodextrin glycosyltransferases (CGT). Since the reproducibility depends mostly on the nature of the substrate, maltotriose was employed. The cyclodextrins formed are measured directly in situ by complexation with methyl orange and by recording the change of absorbance photometrically. The test was successfully used with CGT from Klebsiella pneumoniae M5 al and CGT from alkalophilic Bacillus. [on SciFinder (R)]


Published in:
Proceedings of the First International Symposium on Cyclodextrins, 89-94
Year:
1982
Keywords:
Note:
CAN 98:13412 7-1 Enzymes Inst. Chem. Eng.,Swiss Fed. Inst. Technol.,Lausanne,Switz. Conference written in English. 9030-09-5 Role: BAC (Biological activity or effector, except adverse), BSU (Biological study, unclassified), BIOL (Biological study) (detn. of cyclization activity of); 547-58-0; 1109-28-0 Role: BIOL (Biological study) (in cyclodextrin glycosyltransferase detn.)
Laboratories:




 Record created 2006-04-18, last modified 2018-01-27


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