Since its development in 1986, the Atomic force microscope (AFM) has become a revolutionary tool for surface analysis. AFM is of special interest to biologists because of its ability to operate in liquids. Applications include imaging molecules, cells, tissues, biomaterials and measuring forces. The objective of this project is to fabricate small AFM cantilevers, with high resonance frequency for biological application. The mould and transfer technique with dry release is used to fabricate the cantilevers.