pH Controlled Diffusion of Proteins with Different pI Values across a Nanochannel on a Chip

Electrostatic interactions dominate the diffusion of proteins in a nanochannel, which was measured and modeled for pH values above and below the isoelectric points of three lectin proteins. Maximal diffusion coefficients were obtained when the proteins were neutral at their pI. This pH-controlled transport led to a separation of biomolecules across the nanochannel. A pI shift was observed, due to reversible adsorption of proteins on the walls, which affects the pH in the nanochannel.


Published in:
Nano Letters, 6, 3, 543-547
Year:
2006
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Note: The status of this file is: EPFL only


 Record created 2006-04-10, last modified 2018-03-17

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