pH Controlled Diffusion of Proteins with Different pI Values across a Nanochannel on a Chip

• Published in: Nano Letters, vol. 6, num. 3, p. 543-547
• Publication date: 2006

Electrostatic interactions dominate the diffusion of proteins in a nanochannel, which was measured and modeled for pH values above and below the isoelectric points of three lectin proteins. Maximal diffusion coefficients were obtained when the proteins were neutral at their pI. This pH-controlled transport led to a separation of biomolecules across the nanochannel. A pI shift was observed, due to reversible adsorption of proteins on the walls, which affects the pH in the nanochannel.

Reference

• LMIS4-ARTICLE-2006-004
• doi:10.1021/nl052372h
• View record in Web of Science

Record created on 2006-04-10, modified on 2017-05-10