Protein-Functionalized Polymer Brushes

A new strategy for the prepn. of protein-functionalized polymer brushes is reported, which is based on a combination of surface-initiated atom transfer radical polymn. (ATRP), p-nitrophenyl chloroformate activation of the surface hydroxyl groups, and subsequent O6-benzylguanine (BG) functionalization. The BG-functionalized brushes are used to chemoselectively immobilize O6-alkylguanine-DNA-alkyltransferase (AGT) fusion proteins with a defined orientation and surface d. These protein-modified polymer brushes are attractive candidates for the development of protein microarrays. [on SciFinder (R)]


Published in:
Biomacromolecules, 6, 3, 1602-1607
Year:
2005
Laboratories:




 Record created 2006-02-27, last modified 2018-01-27


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