PPS-PEG surface coating to reduce thrombogenicity of small diameter ePTFE vascular grafts

Aims: Patency failure of small vascular synthetic grafts is still a major problem for coronary and peripheral revascularization. Thus, three new surface coatings of small synthetic grafts were tested in an acute pig model to evaluate their thrombogenicity (extracorporeal arterio-venous shunt) and in a chronic rat model to evaluate the tissue reaction they induced (s.c. implantation). Methods: In five domestic pigs (25-30 kg) an extracorporeal femora-femoral arterio-venous shunt model was used. The study protocol included first a non-heparinized perfusion sequence followed by graft perfusion after 10,000 UI iv heparin. Grafts were perfused for 3 and 9 min. The following coatings were tested on ePTFE grafts: poly-propylene sulfide (PPS) - poly-ethylene glycol (PEG) (wet and dry applications) as well as carbon. Two sets of control were used, one dry and one wet (vehicle only). After perfusion grafts were examd. by SEM for semi-quant. assessment (score 0-3) of cellular and microthrombi deposition. To assess tissue compatibility, pieces of each material were implanted s.c. in 16 Wistar rats. At 2, 4, 8, 12 wk four animals each were sacrificed for semi-quant. (score 0-3) histol. evaluation of tissue reaction. Results: In the pig model, cellular deposition and microthrombi formation increased over time. In non-heparinized animals, the coatings did not improve the surface characteristics, since they did not prevent microthrombi formation and cellular deposition. In heparinized animals, thrombogenicity was lowest in coated grafts, esp. in PPS-PEG dry (p<0.05), and highest in controls. Cell deposition was lowest in PPS-PEG dry, but this difference was not statistically significant vs. controls. In the rat model, no significant differences of the tissue reaction could be shown between materials. Conclusion: While all coatings failed to add any benefit for lowering tissue reaction, surface coating with PPS-PEG (dry application) reduced thrombogenicity significantly (in heparinized animals) and thus appears to be promising for improving graft patency of small synthetic vascular prostheses. [on SciFinder (R)]

Published in:
International Journal of Artificial Organs, 28, 10, 993-1002

 Record created 2006-02-27, last modified 2018-03-17

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