Nucleic acid sequencing by processive degradation of dye-labeled polynucleotides immobilized on filter membranes
The invention relates to a method and a device for sequencing nucleic acid mols. on a perforated membrane. The inventive method and device can be esp. used in multiplex sequencing. The method involves prepg. an oligonucleotide that is labeled to satn. with base-specific fluorescent dyes that is then immobilized on a filter. The labeled sequence is then processively degraded with an exonuclease with the released bases being detected fluorometrically. [on SciFinder (R)]
7709894
Alternative title(s) : (de) Sequenzierung ĂĽber lochmembranen (fr) Sequencage au moyen de membranes perforees (en) Sequencing on perforated membranes
Patent number | Country code | Kind code | Date issued |
US2005130159 | US | A1 | 2005-06-16 |
EP1458890 | EP | A2 | 2004-09-22 |
WO03052136 | WO | A3 | 2004-01-15 |
DE10162535 | DE | A1 | 2003-07-03 |
AU2002358756 | AU | A1 | 2003-06-30 |
AU2002358756 | AU | A8 | 2003-06-30 |
WO03052136 | WO | A2 | 2003-06-26 |