Membrane receptor proteins at interfaces for bioanalytics in the micro- and nanometer range

A review. Cell-based assays are the predominant means of assessing activation of ionotropic receptors and G protein-coupled receptors (GPCRs), but are limited by their inherent complexity. Here we present an approach to immobilize receptors stably and with defined orientation in microarrays on sensor surfaces. Using optical and elec. methods we were able to follow ligand binding, receptor activation and receptor deactivation. Our findings are a novel approach for functional screening of pharmacol. interesting receptor proteins in high d. format. [on SciFinder (R)]

Published in:
Micro Total Analysis Systems, 2001, 242-243
Presented at:
5th mTAS 2001 Symposium, Monterey, CA, United States, Oct. 21-25, 2001

 Record created 2006-02-27, last modified 2018-03-17

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