Journal article

The development and characterization of a high resolution bio-reaction calorimeter for weakly exothermic cultures

A unique bench-scale heat flux calorimeter is described, which is capable of detecting the low heat signal of an animal cell culture (a max. of 200 mW/l is produced with a batch culture of SF9 insect cells). After the tech. modifications that enhanced the sensitivity of a com. heat flux reaction calorimeter, RC1 from Mettler-Toledo AG. Switzerland, the behavior of this system is analyzed here under biol. operation conditions. Not only the heat flows provoked by the bioprocess parameters largely det. the final calorimetric resoln., but they are also more important than the culture heat signal (heat of reaction). Structural modifications aiming at the limitation of the non-biol. heat flows are described such as insulating and thermostatic housings, and glass reactors with a different geometry. The current resoln. of the RC1 is from +-4 to 12 mW/l compared to the former +-50 mW/l [Biotechnol. Bioeng. 57 (1998) 610], under usual animal cell culture conditions. This resoln. is similar to that of microcalorimeters [Biotechnol. Bioeng. 58 (1998) 464; Thermochim. Acta 309 (1998) 63; Thermochim. Acta 332 (1999) 211; J. Biochem. Biophys. Methods 32 (1996) 191] with the advantage of an in situ measurement technique and a well-controlled culture (from the biol. point of view). The monitoring sensitivity and control capacity of this instrument shows a clear potentiality for metabolic studies of low heat effect cell cultures and as a metabolic probe for industrial scale bioreactors's control. [on SciFinder (R)]


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