Ceramic hydroxyapatite: a new tool for separation and analysis of IgA monoclonal antibodies

A novel downstream process is reported for preparative scale prodn. of dimeric IgA, a protein with potential pharmaceutical applications. Time-consuming size exclusion chromatog. was replaced by ceramic hydroxyapatite chromatog. (CHT). A clear baseline sepn. of the dimeric IgA from the polymeric forms using a step-gradient-step protein elution program is described. Application of this method for IgA anal. during prodn. is suggested. [on SciFinder (R)]


Published in:
Animal Cell Technology: From Vaccines to Genetic Medicine, [Proceedings of the Meeting of the ESACT], 14th, Vilamoura, Port., May 1996, 265-269
Year:
1997
Laboratories:




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