Preparation of RNA using triple substituted silyloxymethyl-group as a protection-group of sugar moiety
The ribonucleoside-derivs. serve for the synthesis of ribonucleic acids and comprise a triple substituted silyloxymethyl-group as a protection-group on the oxygen atom in 2'-position. The ribonucleoside-derivs. may be suitably protected on the nucleobase and on the oxygen in 5'-position also. The new protection-groups in 2'-O-position are superior to conventional such protection the groups as they are not subject to isomerization and give higher coupling yields. Ribonucleoside I whereby R1 is a base of the purine- or pyrimidine-family or a deriv. of such a base, R2 is a proton or a substituted deriv. of phosphonic acid, R3 is a proton or a suitable protection-group, R4, R5, R6 are advantageously three identical or different alkyl- or aryl-substituents which together comprise between 6 and 30 carbons atoms. were prepd. and used in the synthesis of RNA. Thus, nucleoside phosphoramidite II was prepd. and used in the synthesis of RNA. [on SciFinder (R)]
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Alternative title(s) : (de) Ribonukleosid-derivat und verfahren zu seiner herstellung (fr) Derive du ribonucleoside et son procede de preparation (en) Ribonucleoside-derivative and method for preparing the same
Patent number | Country code | Kind code | Date issued |
JP4473445 | JP | B2 | 2010-06-02 |
DE69810934 | DE | T2 | 2003-11-27 |
ES2191343 | ES | T3 | 2003-09-01 |
DE69810934 | DE | D1 | 2003-02-27 |
AT231518 | AT | T | 2003-02-15 |
EP1005478 | EP | B1 | 2003-01-22 |
JP2001515087 | JP | A | 2001-09-18 |
EP1005478 | EP | A1 | 2000-06-07 |
US5986084 | US | A | 1999-11-16 |
AU9532198 | AU | A | 1999-03-08 |
WO9909044 | WO | A1 | 1999-02-25 |